Volume 85, Issue 1 p. 113-122
Translational Periodontology

High Concentration but Low Activity of Hepatocyte Growth Factor in Periodontitis

Johanna Lönn

Corresponding Author

Johanna Lönn

The Institution for Protein Environment Affinity Surveys (PEAS Institute), Linköping, Sweden.

Division of Clinical Medicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden.

Correspondence: Dr. Johanna Lönn, Clinical Research Center, University Hospital, SE-701 85 Örebro, Sweden. Fax: +46 19 602 6650; e-mail: [email protected].Search for more papers by this author
Carin Starkhammar Johansson

Carin Starkhammar Johansson

Division of Cardiovascular Medicine, Department of Medical and Health Sciences, Linköping University, Linköping, Sweden.

Centre for Oral Rehabilitation, Public Dental Health Care, County Council of Östergötland, Linköping, Sweden.

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Sravya Nakka

Sravya Nakka

The Institution for Protein Environment Affinity Surveys (PEAS Institute), Linköping, Sweden.

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Eleonor Palm

Eleonor Palm

Division of Clinical Medicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden.

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Torbjörn Bengtsson

Torbjörn Bengtsson

Division of Clinical Medicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden.

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Fariba Nayeri

Fariba Nayeri

The Institution for Protein Environment Affinity Surveys (PEAS Institute), Linköping, Sweden.

Division of Infectious Diseases, University Hospital, Linköping, Sweden.

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Nils Ravald

Nils Ravald

Division of Cardiovascular Medicine, Department of Medical and Health Sciences, Linköping University, Linköping, Sweden.

Centre for Oral Rehabilitation, Public Dental Health Care, County Council of Östergötland, Linköping, Sweden.

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First published: 01 January 2014
Citations: 20

Abstract

Background: High levels of hepatocyte growth factor (HGF), a healing factor with regenerative and cytoprotective effects, are associated with inflammatory diseases, including periodontitis. HGF biologic activity requires binding to its receptors, the proto-oncogene c-Met and heparan sulfate proteoglycan (HSPG). This study investigates HGF expression and its relationship to subgingival microbiota in medically healthy individuals with and without periodontitis.

Methods: Saliva, gingival crevicular fluid (GCF), and blood samples from 30 patients with severe periodontitis and 30 healthy controls were analyzed for HGF concentration using enzyme-linked immunosorbent assay and binding affinity for HSPG and c-Met using surface plasmon resonance. The regenerative effects of saliva from three patients and controls were analyzed in an in vitro model of cell injury. Subgingival plaques were analyzed for the presence of 18 bacterial species.

Results: Patients with periodontitis showed higher HGF concentrations in saliva, GCF, and serum (P <0.001); however, the binding affinities for HSPG and c-Met were reduced in GCF and saliva (P <0.002). In contrast to the controls, saliva from patients showed no significant regenerative effect over time on gingival epithelial cells. Compared with controls, patients had a higher prevalence of periodontally related bacteria.

Conclusions: Higher circulatory HGF levels indicate a systemic effect of periodontitis. However, the HGF biologic activity at local inflammation sites was reduced, and this effect was associated with the amount of periodontal bacteria. Loss of function of healing factors may be an important mechanism in degenerative processes in periodontally susceptible individuals.